Scientists engineer a copper-containing polymer that greatly boosts the antimicrobial activity of hydrogen peroxide

Hydrogen peroxide reacts with copper to produce hydroxyl radicals with strong antibacterial properties. However, this requires high copper concentrations because two copper atoms have to come close together, which occurs by chance. Now, scientists at Tokyo University of Science, Japan, engineered a long polymer with copper-containing side units that create regions with locally high copper density, boosting the antibacterial activity of hydrogen peroxide and paving the way to a new drug design concept.

The discovery of antibiotics was a huge breakthrough in medicine, which helped save countless lives. Unfortunately, their widespread use has led to the rapid evolution of highly resistant bacterial strains, which threaten to take humanity back to square one in the fight against infectious diseases. Even though researchers are seeking new design concepts for antibacterial drugs, the overall development of new agents is currently on the decline.

To tackle this serious problem, scientists at Tokyo University of Science, Japan, are exploring a novel approach to boost the in vivo antibacterial activity of hydrogen peroxide (H₂O₂), a commonly used disinfectant. In a recent study published in Macromolecular Rapid Communications, a team led by Assistant Professor Shigehito Osawa and Professor Hidenori Otsuka reported their success in enhancing H₂O₂ activity using carefully tailored copper-containing polymers.

To understand their approach, it helps to know how H₂O₂ acts against bacteria in the first place, and the role that copper plays. H₂O₂ can be decomposed into a hydroxyl radical (^•OH) and a hydroxide anion (OH−), the former of which is highly toxic to bacteria as it readily destroys certain biomolecules. Copper in its first oxidation state, Cu(I), can catalyze the splitting of H₂O₂ into a hydroxyl radical and a hydroxide anion, turning into Cu(II) in the process through oxidation (Figure 1). Curiously, H₂O₂ can also catalyze the reduction of Cu(II) to Cu(I), but only if this reaction is somehow facilitated. One way to achieve this is to have Cu(II)-containing complexes get close enough together.

However, when using Cu(II)-containing complexes dissolved in a solution, the only way for them to come close together is by accidentally bumping into each other, which requires an excessively high concentration of copper. The team found a workaround to this issue by drawing inspiration from cellular chemistry, as Dr. Osawa explains: "In living organisms, copper forms complexes with proteins to efficiently catalyze redox reactions. For example, tyrosinase has two copper complex sites in close proximity to each other, which facilitates the formation of reaction intermediates between oxygen species and copper complexes. We thought we could leverage this type of mechanism in artificially produced polymers with copper complexes, even if dispersed in a solution."

With this idea, the researchers developed a long polymer chain with dipicolylamine (DPA) as copper-containing complexes. These DPA-copper complexes were attached to the long polymer backbone as "pendant groups." When these polymers are dispersed in a solution, the Cu(II) atoms in the pendant groups are kept in close proximity and locally high densities, vastly increasing the chances that two of them will be close enough to be reduced to Cu(I) by H₂O₂. Through various experiments, the scientists demonstrated that the use of these tailored polymers resulted in higher catalytic activity for the splitting of H₂O₂, resulting in more OH^• even for lower concentrations of copper. Further tests using Escherichia coli cultures showed that these polymers greatly enhanced the antibacterial potential of H₂O₂.

While the results of this study open up a new design avenue for antimicrobial drugs, there may also be useful applications in the food industry as well. "Because copper is an essential nutrient for living organisms, the antibacterial agent developed in this study holds promise as an efficient food preservative, which could contribute to increasing the variety of foods that can be preserved over long shelf times," highlights Dr. Osawa. Let us hope this new strategy makes it easier for us to keep microscopic menaces at bay!

・Figure 1. Generating antibacterial hydroxyl radicals using copper as a catalyst

The proposed polymer, with its backbone shown in blue, creates regions with a high local density of copper side units (pendants). This helps reduce Cu(II) to Cu(I), the most difficult step in the redox reaction shown, to ultimately produce more hydroxyl radicals (^•OH).
Figure courtesy: Assistant Professor Shigehito Osawa

Reference

Title of original paper	:	Accelerated Redox Reaction of Hydrogen Peroxide by Employing Locally Concentrated State of Copper Catalysts on Polymer Chain
Journal	:	Macromolecular Rapid Communications
DOI	:	10.1002/marc.202100274

About The Tokyo University of Science

Tokyo University of Science (TUS) is a well-known and respected university, and the largest science-specialized private research university in Japan, with four campuses in central Tokyo and its suburbs and in Hokkaido. Established in 1881, the university has continually contributed to Japan's development in science through inculcating the love for science in researchers, technicians, and educators.

With a mission of "Creating science and technology for the harmonious development of nature, human beings, and society", TUS has undertaken a wide range of research from basic to applied science. TUS has embraced a multidisciplinary approach to research and undertaken intensive study in some of today's most vital fields. TUS is a meritocracy where the best in science is recognized and nurtured. It is the only private university in Japan that has produced a Nobel Prize winner and the only private university in Asia to produce Nobel Prize winners within the natural sciences field.